SubtiBank SubtiBank
opuAC [2017-11-24 14:03:01]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

opuAC [2017-11-24 14:03:01]

glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)
locus
BSU03000
pI
8.02
mw
32.06 kDa
protein length
293 aa Sequence Blast
gene length
879 bp Sequence Blast
function
compatible solute transport
product
glycine betaine ABC transporter (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    323,119 → 324,000

    The protein

    Catalyzed reaction/ biological activity

  • binding for subsequent uptake of glycine betaine and arsenobetaine [pubmed|29159878,24561588]
  • binding for subsequent uptake of dimethylglycine [Pubmed|24561588]

    • Structure

  • [PDB|2B4L] (complex with glycine betaine), [PDB|3CHG] (in complex with DMSA)

    • [SW|Localization]

  • associated to the membrane (via [protein|5F989C57010ACFC03E37AF5A894153F432520921|OpuAB]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23175650], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

    • regulation

  • induced by osmotic stress [Pubmed|23175650]
    • view in new tab

    Biological materials

    Mutant

  • BKE03000 (Δ[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
  • BKK03000 (Δ[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT

    • Expression vector

  • pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 27935846

    • Original publications

  • 10092453,9335265,16645306,16225868,16445940,7622480,18567662,21296969,22383849,23175650,23646920,24561588,25012968,29159878